EliZyme for End-point RT-PCR

EliZyme™ OneS Kit

To achieve efficient cDNA synthesis and PCR in a single tube the EliZyme™ OneS kit uses the latest developments in reverse transcriptase technology and buffer chemistry. Thermostable reverse transcriptase is active at temperature from 45°C to 55°C for regions containing high secondary structures.  The PCR is performed by EliZyme HS FAST DNA polymerase.

EliZyme HS FAST uses advanced hot-start technology for greater sensitivity. Primer-dimer formation and non-specific amplification from low copy number target sequences are avoided by inactivation below 65°C. DNA polymerase in EliZyme HS FAST is inactivated until the initial activation step at 95°C

The combination of enzyme and buffer system makes exceptional PCR performance on complex templates such as mammalian genomic DNA.

Features:

  • Thermostable reverse transcriptase RTase (45 °C to 55 °C)
  • Advanced RNase inhibitor
  • Antibody-mediated hot start technology for unrivalled detection of low copy number templates
  • High PCR yields under standard and fast PCR conditions
  • Efficient specific amplification from complex templates including GC and AT-rich sequences
  • EliZyme HS FAST polymerase is included

Items Ref. No. Size Package Price ex. VAT VAT  
EliZyme™ OneS Kit EZ4505 1x1,25ml mix + 1x0,125ml transcriptase 50 rxns € 207.50 21 % Buy
EliZyme™ OneS Kit EZ4510 2x1,25ml mix + 2x0,125ml transcriptase 100 rxns € 395.20 21 % Buy
EliZyme™ OneS Kit EZ4550 2x6,25ml mix + 1x1,25ml transcriptase 500 rxns € 1 877.30 21 % Buy

MSDS EliZyme OneS Kit-20x RTase CZ

(published 07.02.2019, size 569.3 kB)

MSDS EliZyme OneS Kit-2x OneS Kit MIX CZ

(published 07.02.2019, size 545.7 kB)

Návod k použití EliZyme OneS Kit

(published 24.08.2023, size 916.9 kB)

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