EliZyme™ Library Quantification Kit
Advantages
- Accurate quantification
- Hot-start technology inactivates the enzyme below 65 °C to prevent primer-dimer formation and non-specific amplification
- For all Real-Time PCR instruments
- Very high sensitivity
Applications
- Quantification of total RNA, mRNA and virus sequences
Accurate quantification of library plays a crucial role in next-generetion sequencing. When pooling libraries, any deviation of concentration between samples can result in variations in the number of sequenced reads.
EliZyme Library Quantification Kit is based on qPCR method. EliZyme Library Quantification Kit for Illumina® contains all the reagents required for successful library quantifiction. Library quantification is performed by amplification of pre-dilluted DNA standards and diluted library samples. Primers recognise the adapter sequences used for Illumina® next-generation sequencing. The average Ct value of each DNA standard is plotted against concentration in pM (in log scale) generating a standard curve used for calculating library concentration.
| Items | Ref. No. | Size | Package | Price ex. VAT | VAT | |
|---|---|---|---|---|---|---|
| EZ4815 | 500/1000 rxns | On request | 21 % | |||
| DNA Standards 1-5 | DNAS | 5 x 80 µl | 1 pack | On request | 21 % |
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