Description of product lines
Product lines of EliGene
EliGene® kits are divided according to detection chemistry to basic lines RT, LC and UNI, respectively.
In RT line, the TaqMan chemistry using hydrolyzing fluorescence probes is used in the combination with hot-start polymerase and ROX dye as a passive reference as an optional additive. The total reaction volume is 20/25 ul when 5 ul of isolated DNA is added directly to ready to use mastermix containing all necessary components for reaction. In RT kits, the fluorescence dyes FAM and HEX and in particular products also TexasRed and Cy5 in combination with non-fluorescent quenchers are used. These kits are designed for Applied Biosystems (ABI 7300, 7500, 7500FAST), Thermofisher Scientific (QS3 and QS5), Roche (LightCycler Nano, LightCycler 480), Bio-Rad (CFX96) instruments, however, can be used with the other instruments that meet the spectral characteristics of used fluorescence dyes. The typical time of the run for RT kits is about 105 minutes.
In LC line, the Molecular Beacons using hybridization fluorescence probes or TaqMan chemistry using hydrolyzing fluorescence probes are used in combination with one-step master mix for reverse transcription with consequent qPCR analysis. The total reaction volume is 20/30 ul when 5/10 ul of isolated RNA is added to mastermix created by mixing of reverse transcriptase with reaction mix containing all necessary components for the reaction. The fluorescence dyes FAM, HEX, TexasRed and Cy5 together with non-fluorescence quenchers are used. The LC kits are designed for the majority of real-time instruments on the market that meet the spectral characteristics of used fluorescence dyes in particular kits. The typical time of run for LC kits is about 70 minutes.
In UNI line, the Molecular Beacons using hybridization fluorescence probes or TaqMan chemistry using hydrolyzing fluorescence probes are used in combination with hot-start polymerase. The total reaction volume is 20 ul when 5 ul of isolated DNA is added directly to ready-to use mastermix containing all necessary components for reaction. In UNI kits the fluorescence dyes FAM and HEX in combination with non-fluorescent quenchers are used. These kits are designed for almost all real-time PCR instruments on the market. The typical time of run for UNI kits is about 60 minutes.
DNA/RNA Isolation
The amplification success depends mostly on the quality of the purified NA. The highest sensitivity in PCR detection can only be achieved when you have first-rate sample preparation. The quality of sample preparation depends not only on isolation procedure but also on timing of collection (clinical relevancy), transport and storage conditions. The presence of conservation agents in transport media could lead to PCR inhibition as well. Taking together all these facts, the isolation procedure must be rigorously tested to avoid false negative results.
EliGene® and MAGNETO isolation kits offer to customer optimized solution with the high quality reagents for manual and automatic preparation. EliGene® and MAGNETO isolation kits are rigorously tested in order to exclude the presence of contaminants and PCR inhibitors. Together with EliGene® detection kits including Internal Control, EliGene® or MAGNETO isolation kits, we provide optimal solution for accurate and high sensitive diagnostics.