EliGene® Anaplasma phagocytophilum UNI

Intended use

EliGene® Anaplasma phagocytophilum UNI is intended for DNA diagnostics of pathogenic species Anaplasma phagocytophilum from isolated DNA.

Principle of the method

This diagnostic kit is based on RealTime PCR method using Molecular Beacons probes. In this kit primers and Molecular Beacons labeled probes (FAM and HEX) for the detection of Anaplasma DNA and internal control are used.

Compatible instruments

The following list represents instruments on which the above mentioned kits were tested. Please contact us for compatibility with other instruments.

• Roche: LightCycler® 480, LightCycler® Nano, LightCycler® 2.0
• Thermofisher Scientific: Quant Studio 3 a 5
• Applied Biosystems: ABI 7500FAST
• Qiagen: RotorGene 6000 nebo Q
• Bio-Rad: CFX96 Touch Real-Time PCR Detection System
• Bio Molecular Systems: MIC qPCR Cycler

Introduction

Anaplasma phagocytophilum is obligate intracellular, gram-negative rickettsial organisms infecting human leukocytes. Anaplasma is transmitted to humans by tick bites primarily from the tick Ixodes scapularis and the western tick Ixodes pacificus. It causes human granulocytic anaplasmosis (HGA) previously known as human granulocytic ehrlichiosis (HGE). Typical symptoms include fever, headache, chills, and muscle aches and they usually occur within 1-2 weeks of a tick bite. The epidemiology of this infection is very much like that of Lyme disease (caused by Borrelia burgdorferi) and babesiosis (caused primarily by Babesia microti), which all have the same tick vector.

Clinical material and recommended DNA isolation procedure

Blood

Manual: EliGene Urine Isolation Kit
Automatic: MAGNETO BodyFluid DNA/RNA Isolation Kit (for equipment ZEPHYRUS Magneto)

CE IVD