EliGene® Chlamydophila pneumoniae UNI (RUO)

Intended use

EliGene^® Chlamydophila pneumoniae UNI is intended for DNA diagnostics of pathogenic species Chlamydophila pneumoniae from isolated DNA sample. For research use only.

Principle of the method

This diagnostic kit is based on RealTime PCR method using Molecular Beacons hybridization probes. In this kit for the detection of Chlamydophila pneumoniae and internal control primers and Molecular Beacons labeled probes (FAM and HEX) are used.

Compatible instruments

The following list represents instruments on which the above mentioned kits were tested. Please contact us for compatibility with other instruments.

• Roche: LightCycler® 480, LightCycler® Nano, LightCycler® 2.0
• Qiagen: RotorGene 6000 nebo Q
• Applied Biosystems: ABI 7500FAST

Introduction

Chlamydophila pneumoniae is an obligate intracellular pathogen. It is a member of the Chlamydiae, a diverse range of obligate intracellular bacteria that includes parasites of amoebae, fish, reptiles, mammals, and humans. Today C. pneumoniae is associated with a remarkably wide range of chronic diseases as meningoencephalitis, arthritis or myocarditis and it is the second most often cause of community-acquired pneumonia. C. pneumoniae spreads by drop infection but in the form of infection elementary bodies (EB) resistant to environmental stresses, it can persist on the surfaces or be spreaded by the aerosol. During the life about 80% of population is infected by C. pneumoniae however in majority of cases the infection is asymptomatic. Some forms of pneumonia can be very serious but in these cases is not clear if C. pneumoniae is not only co-pathogen.

Primary sample collection, handling and storage

Clinical material and recommended DNA isolation procedure:
Throat swabs, Nasopharyngeal aspirates, Sputum     

Manual: EliGene® Urine Isolation Kit
Automatic: MAGNETO BodyFluid DNA/RNA Isolation Kit (for equipment ZEPHYRUS Magneto)

Research Use Only (RUO)