EliGene® MTB UNI

Intended use

EliGene® MTB UNI Kit is qualitative in vitro diagnostic device intended for the detection of bacteria Mycobacterium tuberculosis in clinical material or bacterial cultures.

Principle of the method

This diagnostic kit is based on RealTime PCR method using Molecular Beacons hybridization probes. In this kit for the detection of MTB DNA and internal control primers and Molecular Beacons labeled probes (FAM and HEX) are used.

Compatible instruments

The following list represents instruments on which the above mentioned kits were tested. Please contact us for compatibility with other instruments.

• Roche: LightCycler® 480, LightCycler® Nano, LightCycler® 2.0
• Thermofisher Scientific: Quant Studio 3 a 5
• Applied Biosystems: ABI 7500FAST
• Qiagen: RotorGene 6000 nebo Q
• Bio-Rad: CFX96 Touch Real-Time PCR Detection System
• Bio Molecular Systems: MIC qPCR Cycler

Introduction

Genus Mycobacterium comprises more than 50 species. Among them obligatory pathogenic etiological agents of tuberculosis and leprosy as well as the facultative pathogenic and non-pathogenic species representing important part of naturally biotopes can be found. M. tuberculosis comprises to the one complex with genetically close related M. bovis and it is etiological agents of human tuberculosis. M. bovis is etiological agents of cattle, pets and wild beast tuberculosis and rarely of human tuberculosis.

Clinical material and recommended DNA isolation procedure

Sputum, BAL, exudates

Manual: EliGene® MTB Isolation Kit
Automatic: MAGNETO BodyFluid DNA/RNA Isolation Kit (for equipment ZEPHYRUS Magneto)

Urine

Manual: EliGene® Urine Isolation Kit
Automatic: MAGNETO BodyFluid DNA/RNA Isolation Kit (for equipment ZEPHYRUS Magneto)

CE IVD