EliGene® STD NEI/TRI RT

Intended use

EliGene® STD NEI/TRI RT kit is intended for DNA diagnostic of pathogenic species Neisseria gonorrhoeae and Trichomonas vaginalis from isolated DNA sample.

Principle of the method

This diagnostic kit is based on RealTime PCR method. In this kit primers and dual labeled fluorescent probes for the detection of DNA are used.

Compatible instruments

The following list represents instruments on which the above mentioned kits were tested. Please contact us for compatibility with other instruments.

• Thermofisher Scientific: Quant Studio 5
• Bio-Rad: CFX96 Touch Real-Time PCR Detection System
• Bio Molecular Systems: MIC qPCR Cycler

Introduction

Neisseria gonorrhoeae (gonococci) is gram-negative, non-motile and non-sporulated diplococcic. Of the eleven species of Neisseria colonizing humans, only two are pathogens, N. gonorrhoeae and N. meningitidis. N. gonorrhoeae is the causative agent of sexual transmitted disease gonorrhea which is the third most common sexual disease, worldwide is registered about 62.2 million cases of gonorrhea. In males and females the gonorrhea is clinically manifested as urethritis with a purulent (or pus-like) discharge from the genitals, in females also as cervicitis. Complication can be infection in pelvic region leading up to infertility in females. The classical penicillin family of antibiotics is relatively well effective in therapy, but the number of resistance strains is increasing. Also the simultaneous infection by Chlamydia infection is common. For DNA diagnostic purposes the urine, urethral swab or vaginal swab are recommended.

Trichomonas vaginalis is an anaerobic parasitic protozoan, that causes trichomoniasis, a sexually transmitted disease. There are about 30 million infections in sub-Saharan Africa each year. Based on a systematic review of articles published in recent years, estimated prevalence of Trichomonas vaginalis in Europe is 0.2 % in men and 1.6 % in women. The infection is asymptomatic in about 50 % of women. However, when symptoms appear, women suffer for vaginal discharge, pruritus or dysuria, vaginitis and cervicitis. In men, symptoms include urethritis, prostatitis and decreased fertility. For DNA diagnostic purposes the urine, urethral swab or vaginal swab are recommended.

Clinical material and recommended DNA isolation procedure

Urine, swabs

Manual: EliGene® Urine Isolation Kit (ELISABETH PHARMACON)

Automatic: ZEPHYRUS Magneto (ELISABETH PHARMACON)

CE IVD