EliGene® VZV UNI

Intended use

EliGene® VZV RT Kit is intended for the detection and quantification of Varicella zoster virus (VZV) in clinical samples.

Principle of the method

This diagnostic kit is based on RealTime PCR method. In this kit primers and labeled probes (FAM and HEX) for the detection of VZV DNA and for the detection of internal control are used.

Introduction

VZV is a double stranded DNA virus of the Herpes group. During VZV infection there can be two different manifestations of the disease - chickenpox or shingles. The manifestations include rash, low grade fever and malaise. Varicella may rarely result in complications including VZV encephalitis or pneumonia. VZV is certainly transmissible through the airborne route and does not require close personal contact. The skin lesions are certainly full of infectious virus particles. After an incubation period of 14 days, the virus arrives at its main target organ, the skin. The virus can survive in the human body in the latent form for a long time.
Kit EliGene® VZV RT has a very high sensitivity that allows detecting 10 genomic DNA in MasterMix.

Compatible instruments

The following list represents instruments on which the above mentioned kits were tested. Please contact us for compatibility with other instruments.

• Roche: LightCycler® 480, LightCycler® Nano, LightCycler® 2.0
• Thermofisher Scientific: Quant Studio 3 a 5
• Applied Biosystems: ABI 7500FAST
• Qiagen: RotorGene 6000 nebo Q
• Bio-Rad: CFX96 Touch Real-Time PCR Detection System
• Bio Molecular Systems: MIC qPCR Cycler

Primary sample collection, handling and storage

Serum, plasma, CSF, swabs

Manual: EliGene Urine Isolation Kit (ELISABETH PHARMACON)
Automatic: MAGNETO BodyFluid DNA/RNA Isolation Kit (for equipment ZEPHYRUS Magneto)

Tissue, biopsies

Manual: UltraClean Tissue DNA Kit (Qiagen), chemagic DNA Tissue10 Kit (Perkin Elmer)
Automatic: Prepito DNA Tissue10 Kit (Perkin Elmer)

CE IVD