EliZyme™ OneS Kit

To achieve efficient cDNA synthesis and PCR in a single tube the EliZyme™ OneS kit uses the latest developments in reverse transcriptase technology and buffer chemistry. Thermostable reverse transcriptase is active at temperature from 45°C to 55°C for regions containing high secondary structures.  The PCR is performed by EliZyme HS FAST DNA polymerase.

EliZyme HS FAST uses advanced hot-start technology for greater sensitivity. Primer-dimer formation and non-specific amplification from low copy number target sequences are avoided by inactivation below 65°C. DNA polymerase in EliZyme HS FAST is inactivated until the initial activation step at 95°C

The combination of enzyme and buffer system makes exceptional PCR performance on complex templates such as mammalian genomic DNA.

Features:

• Thermostable reverse transcriptase RTase (45 °C to 55 °C)
• Advanced RNase inhibitor
• Antibody-mediated hot start technology for unrivalled detection of low copy number templates
• High PCR yields under standard and fast PCR conditions
• Efficient specific amplification from complex templates including GC and AT-rich sequences
• EliZyme HS FAST polymerase is included