EliZyme™ FAST Taq

Advantages

• Polymerase speed up to 1 s/kb for amplicons shorter than 1 kb
• Extension 15 s/kb
• Shortens the length of PCR programs
• Adjust the volume of enzyme

Applications

• Routinne PCR
• Sanger sequencing
• GC-rich and AT-rich sequences
• Other genotyping applications (including downstreamh applications)
• TA cloning

EliZyme FAST Taq is a robust enzyme suited for everyday PCR applications including genotyping, screening and library construction. It is characterized by enhanced PCR speed, yield and specificity. The combination of enzyme and buffer system makes exceptional PCR performance on complex templates such as mammalian genomic DNA. EliZyme FAST Taq DNA Polymerase has 5’-3’ exonuclease activity, but no 3’-5’ exonuclease (proofreading) activity. The error rate is approximately 1 error per 2.0 x 10⁵ nucleotides incorporated. PCR products may be cloned into TA cloning vectors because EliZyme FAST Taq DNA polymerase generates A-tailed PCR products.

For higher comfort is EliZyme FAST Taq DNA polymerase also available in a 2x ready mix version. EliZyme FAST Taq MIX Red contains a red dye for tracking during agarose gel electrophoresis. It is suitable for direct loading onto agarose gel.