EliZyme™ HS FAST

Advantages

• Hot-start technology inactivates the enzyme below 65 °C to prevent primer-dimer formation and non-specific amplification
• Polymerase speed up to 1 s/kb for amplicons shorter than 1 kb
• Extension 15 s/kb
• Shortens the length of PCR programs
• Adjust the volume of enzyme

Applications

• Routinne PCR
• Multiplex PCR
• Direct PCR (from bacterial culture, blood, urine)
• Sanger sequencing
• GC-rich and AT-rich sequences
• Other genotyping applications (including downstreamh applications)
• TA cloning

EliZyme HS FAST uses advanced hot-start technology for greater sensitivity. Primer-dimer formation and non-specific amplification from low copy number target sequences are avoided by inactivation below 65°C. DNA polymerase in EliZyme HS FAST is inactivated until the initial activation step at 95°C. The combination of enzyme and buffer system make exceptional PCR performance on complex templates such as mammalian genomic DNA.

For higher comfort is EliZyme HS FAST DNA polymerase also available in a 2x ready mix version. EliZyme HS FAST MIX Red contains a red dye for tracking during agarose gel electrophoresis. It is suitable for direct loading onto agarose gel.