EliGene® Mycoplasma hom/gen UNI

Intended use

EliGene® Mycoplasma hom/gen UNI is intended for DNA diagnostics of pathogenic species Mycoplasma hominis and Mycoplasma genitalium from isolated DNA sample.

Principle of the method

This diagnostic kit is based on RealTime PCR method using Molecular Beacons hybridization probes. In this kit for the detection of Mycoplasma hominis and genitalium DNA and internal control primers and Molecular Beacons labeled probes (FAM and HEX) are used.

Compatible instruments

The following list represents instruments on which the above mentioned kits were tested. Please contact us for compatibility with other instruments.

• Applied Biosystems: ABI 7500FAST
• Roche: LightCycler® 480, LightCycler® Nano, LightCycler® 2.0
• Qiagen: RotorGene 6000 nebo Q
• Thermofisher Scientific: Quant Studio 3 a 5
• Bio-Rad: CFX96 Touch Real-Time PCR Detection System
• Bio Molecular Systems: MIC qPCR Cycler

Introduction

Mycoplasma is bacterium belonging to the family Mycoplasmataceae which is comprised of group of microorganisms that cause respiratory and genital tract infection. These bacteria are detected in about 70% of sexually active people.
Mycoplasma hominis is associated with inflammatory diseases in a pelvis region, bacterial vaginitis, post-natal fever, sepsis and infections of central nerve system with serious consequences. This bacterium is also presumed to play a role during the development of woman infertility even if a literature and studies are not homogenous.
Mycoplasma genitalium is the main cause of non-Chlamydial and non-gonococcal urethritis (NCNGU) in men and cervicitis in women. M. genitalium bacteria infections have been associated also with inflammatory diseases in a pelvis region but this role has not been confirmed.

Primary sample collection, handling and storage

Clinical material and Recommended DNA isolation procedure:
Urine, swabs

Manual: EliGene® Urine Isolation Kit
Automatic: MAGNETO BodyFluid DNA/RNA Isolation Kit (for equipment ZEPHYRUS Magneto)

CE IVD