EliGene® Adenovirus RT

Intended use

EliGene® Adenovirus RT kit is qualitative in vitro diagnostic device intended for the detection of adenovirus in clinical material.

Principle of the method

This diagnostic kit is based on RealTime PCR method. In this kit primers and TaqMan labeled probes (FAM and YakimaYellow) for the detection of ADV DNA and internal control are used.

Compatible instruments

The following list represents instruments on which the above mentioned kits were tested. Please contact us for compatibility with other instruments.

• Applied Biosystems: ABI 7000, 7300, 7500FAST
• Roche: LightCycler® 480, LightCycler® Nano
• Qiagen: RotorGene 6000 nebo Q

Primary sample collection, handling and storage

Serum, plasma, CSF, swabs

Manual: EliGene® Urine Isolation Kit (ELISABETH PHARMACON)
Automatic: MAGNETO BodyFluid DNA/RNA Isolation Kit (for equipment ZEPHYRUS Magneto)

Tissue, Biopsies

Manual: UltraClean Tissue DNA Kit (MoBio), Chemagic DNA Tissue10 Kit (Perkin Elmer)
Automatic: Prepito DNA Tissue10 Kit (Perkin Elmer)

Feces

Manual: UltraClean Fecal DNA Kit (MoBio)

Introduction

Adenoviruses represent the largest non-enveloped viruses. They are able to be transported through the endosome (i.e. envelope fusion is not necessary). They are about 70 to 90 nm in size, nonenveloped icosahedral viruses which are made from up to 252 capsomeres containing double-stranded DNA. The replication cycle of adenovirus takes about 36 hours and newly produced virions are released from the cell as a result of virally induced cell lysis.
Infection is usually transmitted by droplets of respiratory or ocular secretions, by alimentary transfer or sexual intercourse and by the contact with contaminated things or water (virus is able to live out of body at temperature 20°C and lower for a few weeks). Adenoviruses are excreted in feces and go to the waste water and rivers where they live for a long time. The places of the first reproduction are the most often cells of epithelial conjunctiva, nasopharynx and intestine.
Adenovirus infection is clinically manifested by the fever, upper respiratory tract infections, tonsillitis, laryngitis, bronchitis and pneumonia. Conjunctivitis can be sometimes the dominant symptom, especially when the infection is acquired from the contaminated water during the bath. There can develop urethritis in the course of sexual intercourse. In infants and children, adenoviruses (types 40 and 41) usually cause heavy infections in the intestinal tract.
Adenoviral DNA diagnostics can be carried out from feces, urine, cerebrospinal fluid and swab from cornea, conjunctiva, urethra, cervix, rectum and nasopharynx.

CE IVD