EliGene® HCV (RUO)

Intended use

The EliGene® HCV LC kit is intended for detection and quantification of hepatitis C virus RNA. For research use only.

Principle of the method

This detection kit is based on RealTime PCR method. In this kit primers and labeled probes (FAM and HEX) for the detection of HCV RNA and for the detection of internal control are used.

Compatible instruments

The following list represents instruments on which the above mentioned kits were tested. Please contact us for compatibility with other instruments.

• Applied Biosystems: ABI 7500FAST
• Roche: LightCycler® 480, LightCycler® Nano, LightCycler® 2.0
• Qiagen: RotorGene 6000 nebo Q
• Bio-Rad: CFX96 Touch Real-Time PCR Detection System
• Bio Molecular Systems: MIC qPCR Cycler

Clinical material and recommended DNA isolation procedure

Clinical material: Serum, plasma

Manual:

• Chemagic Viral DNA/RNA Kit (Chemagen-PerkinElmer)
• QIAamp DSP Virus Kit(Qiagen)

Automatic:

• MAGNETO BodyFluid DNA/RNA Isolation Kit (for equipment ZEPHYRUS Magneto)

Introduction

Hepatitis C virus is enveloped RNA virus classified to Flaviviridae family. Virus genome consists of 9379 nucleotides. HCV represents heterogeneous group of viruses divided into 6 genotypes (groups) and more than 40 sub-types. EliGene® HCV LC Kit gives the positive results with genotypes 1a, 1b, 2a, 2b, 3a, 4a, 6 and 6b.
Today, the word prevalence about 175 milions persons infected by hepatitis C virus is assessed. Virus is disseminating mainly by parenteral way, the sexual transfer is observed rare. In our conditions, significantly risk group is represented by the injection drug consumers.

For research only