EliGene® HSV1/HSV2 UNI

Intended use

Kit EliGene® HSV1/HSV2 UNI is intended for detection of pathogenic species of herpesvirus 1 (Herpes simplex virus 1 – HSV1) and herpesvirus 2 (Herpes simplex virus 2 – HSV2) in clinical samples.

Principle of the method

This diagnostic kit is based on RealTime PCR method. In this kit primers and labeled probes (FAM and JOE) for the detection of HSV1/HSV2 DNA and for the detection of internal control are used.

Compatible instruments

The following list represents instruments on which the above mentioned kits were tested. Please contact us for compatibility with other instruments.

• Applied Biosystems: ABI 7500FAST
• Roche: LightCycler® 480, LightCycler® Nano, LightCycler® 2.0
• Qiagen: RotorGene 6000 nebo Q
• Thermofisher Scientific: Quant Studio 3 a 5
• Bio-Rad: CFX96 Touch Real-Time PCR Detection System
• Bio Molecular Systems: MIC qPCR Cycler

Primary sample collection, handling and storage

Serum, plasma, CSF, swabs

Manual: EliGene® Urine Isolation Kit (ELISABETH PHARMACON)
Automatic: MAGNETO BodyFluid DNA/RNA Isolation Kit (for equipment ZEPHYRUS Magneto)

Tissue, biopsies

Manual: UltraClean Tissue DNA Kit (MO BIO), chemagic DNA Tissue10 Kit (Perkin Elmer)
Automatic: Prepito DNA Tissue10 Kit (Perkin Elmer)

General iformation

Herpesvirus is enveloped DNA virus. To date, at least eight different herpesvirus species are known to infect man.
HSV1 is enveloped DNA virus. Today, at least eight different herpesvirus species are known to infect man. A primary infection typically occurs during the childhood. The primary infection goes often through symptomless. The HSV1 infection can manifest as an undifferentiational catarrh of respiratory system of adults or older children. The infection of the conjunctiva and cornea manifests as keratoconjunctivitis. HSV1 is a neurotropic virus. The DNA diagnostics of HSV1 is applicable for samples (swabs) from cornea, conjunctiva, skin lesions and blood or from the cerebrospinal fluid.
HSV2 causes genital herpes, characterized by sores in the area of the genitalia. Genital herpes is a sexually transmitted disease. The main symptoms are herpes ulcers in glans penis in men. Lesions in woman are localized in cervix, vagina or perianal area. The activation of the latent infection can produce asymptomatic releasing of virus in cervical secret. HSV2 can cause infection of the brain (encephalitis) in the case of severely defective or compromised immune system. Clinical samples for the DNA diagnostics of HSV2 include samples (swabs) from skin/mucosa lesions, blood or from the cerebrospinal fluid (CSF).

CE IVD