EliZyme for isothermal amplification

Isothermal amplification is a method that allows amplification of DNA or RNA at a constant temperature without the need for thermal cycling. It takes advantage of DNA polymerases (e.g. Bst polymerase) that have high strand displacement capabilities during complementary strand synthesis, thus eliminating the need for high temperature denaturation of DNA.

Isothermal methods include multiple displacement amplification, whole genome amplification and loop-mediated isothermal amplification (LAMP).

The EliZyme™ LAMP product line provides a complete solution for users using isothermal amplification. Based on a recombinant form of an enzyme derived from a large fragment of Bst DNA polymerase, it offers rapid and sensitive amplification of target DNA and RNA at a constant temperature of 65 °C.

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  EliZyme™ LAMP

  EliZyme™ LAMP product line represents a complete solution for the users utilizing an isothermal amplification. EliZyme™ LAMP contain a recombinant enzyme derived from the large fragment of Bst DNA polymerase, possesses strong strand-displacing 5'-3' polymerase activity. 

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  EliZyme™ LAMP OneS MIX

  EliZyme™ LAMP OneS MIX is an innovative enzyme system that enables reverse transcription of target RNA and subsequent fast and sensitive isothermal amplification in a single step. This kit combines Bst Polymerase, known for its strong strand displacement capabilities, with the highly active modified M-MLV Reverse Transcriptase.

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