EliZyme™ LAMP OneS MIX

EliZyme™ LAMP OneS MIX is an innovative enzyme system that enables reverse transcription of target RNA and subsequent fast and sensitive isothermal amplification in a single step. This kit combines Bst Polymerase, known for its strong strand displacement capabilities, with the highly active modified M-MLV Reverse Transcriptase.

By harnessing the robust strand displacement activity of Bst Polymerase, DNA synthesis can occur at a constant temperature (65 ˚C) without the need for thermal cycling. Derived from the large fragment of Geobacillus stearothermophilus (formerly Bacillus stearothermophilus) DNA Polymerase, this specific portion of the protein catalyzes the synthesis of DNA in the 5'-3' direction but lacks the 5'-3' exonuclease domain.

Reverse transcription of target RNA is accomplished through the utilization of the thermostable and highly active RTase, which is combined with an RNase inhibitor to protect the RNA from degradation caused by contaminating RNase enzymes.

The EliZyme™ LAMP OneS MIX delivers rapid and consistent results, demonstrating its effectiveness across a wide range of sample types. The buffer chemistry incorporated in the kit ensures high yield and performance, even under challenging conditions such as the presence of inhibitors.

Using EliZyme™ LAMP OneS MIX is straightforward, as it only requires the addition of primers, template, and water. Additionally, the kit includes a separate tube of fluorescent dye, allowing real-time detection using any qPCR instrument.

Features:

• Includes strand-displacing Bst Polymerase in a 2X mix format
• Supplied with RTase and RNase inhibitor
• Reaction is carried out at a constant temperature (65 ˚C)
• Gives rapid and consistent amplification across a wide range of templates
• Includes an advanced buffer system for higher yield under difficult conditions
• Supplied with a fluorescent dye for real-time detection
• 30-minute protocol

Applications:

• Multiple displacement amplification
• Isothermal amplification
• Loop mediated isothermal amplification (LAMP)
• Molecular diagnostics
• Field diagnostics